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proc mixed procedure of sas studio  (SAS institute)


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    SAS institute proc mixed procedure of sas studio
    Proc Mixed Procedure Of Sas Studio, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mixed+procedure/10__22175_slash_mmb__15701-96-14-16?v=SAS+institute
    Average 90 stars, based on 1 article reviews
    proc mixed procedure of sas studio - by Bioz Stars, 2026-06
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    Generations of FFA-secreting Synechococcus strains. a Schematic representation of the aas locus in the wild-type and engineered strains. The sacB - kan r cassette, previously integrated into the aas locus of the original strain ( Δaas:: sacB - kan r ), was replaced by the indicated DNA constructs using the in-fusion method to generate the respective FFA-secreting strains. b Confirmation by PCR of the complete replacement of the sacB - kan r cassette at the aas locus in each engineered strain. Genomic DNA was amplified using primers flanking the integration site (F1 and R1). Lanes 1–6: size markers, Δaas , Δaas::lipB, Δaas::lipB-rndAB , Δaas::lipC-rndAB , and Δaas::lipC-lipB-rndAB strains, respectively. c Relative levels of transcripts of the rndA1B1 , lipB , and lipC genes in the engineered strains, as determined by quantitative RT-PCR. Transcript levels were normalized by reference to the transcript of the prs gene. Values represent the mean + S.D. (bar) of biological triplicates ( n = 3). Asterisks indicate statistically significant differences between strains (* p < 0.05, ** p < 0.01; one-way ANOVA)

    Journal: Biotechnology for Biofuels and Bioproducts

    Article Title: Overexpression of endogenous galactolipases and an efflux transporter enhances the secretion of extracellular free fatty acids by Synechococcus elongatus PCC 7942

    doi: 10.1186/s13068-026-02768-0

    Figure Lengend Snippet: Generations of FFA-secreting Synechococcus strains. a Schematic representation of the aas locus in the wild-type and engineered strains. The sacB - kan r cassette, previously integrated into the aas locus of the original strain ( Δaas:: sacB - kan r ), was replaced by the indicated DNA constructs using the in-fusion method to generate the respective FFA-secreting strains. b Confirmation by PCR of the complete replacement of the sacB - kan r cassette at the aas locus in each engineered strain. Genomic DNA was amplified using primers flanking the integration site (F1 and R1). Lanes 1–6: size markers, Δaas , Δaas::lipB, Δaas::lipB-rndAB , Δaas::lipC-rndAB , and Δaas::lipC-lipB-rndAB strains, respectively. c Relative levels of transcripts of the rndA1B1 , lipB , and lipC genes in the engineered strains, as determined by quantitative RT-PCR. Transcript levels were normalized by reference to the transcript of the prs gene. Values represent the mean + S.D. (bar) of biological triplicates ( n = 3). Asterisks indicate statistically significant differences between strains (* p < 0.05, ** p < 0.01; one-way ANOVA)

    Article Snippet: Various constructs were generated using the In-Fusion ® Snap Assembly method (Takara Bio Inc., Shiga, Japan) with specific forward and reverse primers (listed in Table S1) to replace the sacB - kan r cassette.

    Techniques: Construct, Amplification, Quantitative RT-PCR